Assalamualaikum guys, hello welcome back to this blog. Hari ini mau berbagi ilmu niih. Ada yang tau nggak apa itu serum free media? Kalau belum so simak info ini yaa
Serum free media are designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free a possible of confounding variables.
Advantages of using serum-free media:
• Increased definition.
• More consistent performance.
• Easier purification and downstream processing.
• Precise evaluations of cellular function.
• Increased growth and/or productivity.
• Better control(s) over physiological responsiveness.
• Enhanced detection of cellular mediators.
Disadvantages of serum free media:
- Cell proliferation is very low
- Cultured cells may need more than one type of media to obtain desired cell culture products.
- greater control over the pH, Temperature, etc. is necessary as compared to that with serum containing mediaa.
- Growth rate and the maximum cell density attained are lower than those with serum containing media.
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